Use the hole borer and bore out pieces of potato then cut into 2cm lengths. In the highly alkaline range, a loss of brightness occurs with additional sodium hydroxide. The active site of the enzyme is specifically shaped to only accept one substrate meaning that only one or very few molecules can be broken down by any one type of enzyme.
The liberation of oxygen and energy in the decomposition has dangerous side-effects. In aqueous solution it rapidly decomposes to form oxygen gas and chromium salts. The accuracy of my results seem high as they correspond to other results although improvements could be made by making sure that the level of hydrogen peroxide is accurate and more repeats are taken.
The perhydroxyl anion acts as a nucleophilic bleaching agent. The more substrate molecules present, the more collisions happen, and more enzyme activity until all the active sites are full causing the reaction to slow down.
The 3d shape of the enzyme molecule is important, as if the shape is changed, the enzyme cannot bond with the substrate.
Higher brightness is obtained with increasing dose of hydrogen peroxide. There are procedures in place to prevent these problems, wearing eye protection and washing hands after the use of hydrogen peroxide.
Johnson This is where another molecule can bind with the enzyme. This is because the more surface area of catalase the more active sites available to bind with the substrate Hydrogen peroxide, meaning more collisions breaking down the substrate faster. My data shows that what I predicted to be true, as you increased the concentration of hydrogen peroxide the time taken for the filter paper to reach the top of the test tube is smaller.
I could do further work by changing the independent variable changing the pH. When the substrate binds with the enzyme, it converts the substrate into one or more products.
An acid wash at pH 2 is found to be effective in removing transition metal ions for chemical pulp bleaching. Most deinking plants take advantage of the synergistic effects of flotation and bleaching by adding hydrogen peroxide during pulping and during dispersion or post-bleaching.
I predict the more you increase the surface area of Catalase, the more oxygen produced, because the hydrogen peroxide has more surface area to collide with, causing a faster rate of reaction.
Perform the experiment three times Two of these are temperature and pH. Below is a diagram explaining the lock and key analogy. Hydrogen peroxide is very effective for improving the brightness of DIP. I would use a different method of using the enzyme.
However, according to Renders a,bthis approach does not appear to be suitable for secondary fibres because filler material such as calcium carbonate can greatly increase the acid demand.
The easiest way to detect poor hydrogen peroxide efficiency is to determine, in parallel with the brightness, the hydrogen peroxide consumption. Record your results 6. The bleaching result is sufficient for many applications using DIP to satisfy the optical requirements of the paper produced.
We will write a custom essay sample on The effect of catalase on hydrogen peroxide or any similar topic specifically for you Do Not Waste HIRE WRITER There will be an optimal point when all the active sites are filled, at this point the reaction will stop, as all the hydrogen peroxide will have been broken down leaving only Catalase, water and oxygen.
The required amount of sodium hydroxide to obtain the maximum bleaching effect also increases. After conducting extensive research into the factors affecting enzyme activity I discovered that there were several different factors, four of which I could feasibly investigate in the school laboratory.
Catalase-forming micro-organisms are killed by the application of peracetic acid, and catalase formation is also inhibited.
Enzymes are specific to their substrate, because the shape of their active site will only fit the shape of their substrate. To combat this the enzyme catalase is used to break down toxic hydrogen peroxide into harmless oxygen and water.
Sodium dithionite is sometimes used in combination with hydrogen peroxide in a two-stage bleaching of DIP for colour removal.
This is known as the lock and key theory. The reliability of my results are good as they are shown to be accurate looking at other data that has been accumulated. Bovin and Blechschmidt and Ackermann have recommended separate bleaching with hydrogen peroxide after a deinking process flotation or washing.
Set up equipment as shown above 3. Higher consistency gives a higher relative concentration of bleaching chemicals that come into direct contact with the fibres, and the displacement of the aqueous phase eliminates dissolved matter that can unfavourably affect the bleaching reaction.
Enzymes do this by binding onto the substrate with the active site of the enzyme. One piece could have had twice as much as another piece. This locks the substrate even more tightly to the enzyme. Empty and rinse conical flask.Hydrogen peroxide - Sample Essay Introduction Enzymes are types of proteins that exist in living organisms to catalyse reactions which break down the enzyme’s substrate into its component parts.
Enzymes do this by binding onto the substrate with the active site of the enzyme. Hydrogen Peroxide Essay Words | 3 Pages. containing more than about 8 percent hydrogen peroxide are corrosive to the skin. First recognized as a chemical compound inhydrogen peroxide is the simplest member of the class of s.
Of the several processes of manufacture, the principal ones involve reactions of oxygen from the air. This will be repeated the same way except for the Hydrogen Peroxide which is increased by 10 cm3 each time and decreasing the water by 10 cm3 each time. We will write a custom essay sample on Hydrogen Peroxide specifically for you.
More Essay Examples on Effect Rubric. In this experiment I boiled the enzyme “catalase” to denature it so it wouldn’t be able to function properly.
I then tested this enzyme by dipping the filter paper in it and then into the concentration of 2% hydrogen peroxide. 2h_2 o_2→o_2+〖2h〗_2 o This process is called decomposition as two hydrogen peroxide molecules are broken down into oxygen and two water molecules.
Catalase is an important enzyme that protects cells from oxidative damage, which hydrogen peroxide can cause.
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